SERINE/THREONINE PROTEIN KINASE
Gene Name - StkP
Cell Location - StkP is located in the cell membrane and is a single-pass transmembrane serine/threonine kinase protein [1][2].
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FUNCTION
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StkP plays a role in the signalling and regulating of gene expression, with its importance in this process being widely recognised.
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It plays major roles in cell wall synthesis and cell division [1] [3].
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StkP helps to control the formation of new cell walls between two daughter cells during cell division by signalling cell wall status information to other key proteins involved in cell division.
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It also functions as an aid in other complex processes within the cell such as cellular metabolism, fitness, and aiding in in the introduction of foreign DNA into the cell by signalling transduction pathways.
STRUCTURE
Click on the image for more information on the secondary and tertiary structure of Serine/Threonine Protein Kinase.
PROTEIN SEQUENCE
10 20 30 40 50
MIQIGKIFAG RYRIVKQIGR GGMADVYLAK DLILDGEEVA VKVLRTNYQT
60 70 80 90 100
DPIAVARFQR EARAMADLDH PHIVRITDIG EEDGQQYLAM EYVAGLDLKR
110 120 130 140 150
YIKEHYPLSN EEAVRIMGQI LLAMRLAHTR GIVHRDLKPQ NILLTPDGTA
160 170 180 190 200
KVTDFGIAVA FAETSLTQTN SMLGSVHYLS PEQARGSKAT VQSDIYAMGI
210 220 230 240 250
IFYEMLTGHI PYDGDSAVTI ALQHFQKPLP SVIAENPSVP QALENVIIKA
260 270 280 290 300
TAKKLTNRYR SVSEMYVDLS SSLSYNRRNE SKLIFDETSK ADTKTLPKVS
310 320 330 340 350
QSTLTSIPKV QAQTEHKSIK NPSQAVTEET YQPQAPKKHR FKMRYLILLA
360 370 380 390 400
SLVLVAASLI WILSRTPATI AIPDVAGQTV AEAKATLKKA NFEIGEEKTE
410 420 430 440 450
ASEKVEEGRI IRTDPGAGTG RKEGTKINLV VSSGKQSFQI SNYVGRKSSD
460 470 480 490 500
VIAELKEKKV PDNLIKIEEE ESNESEAGTV LKQSLPEGTT YDLSKATQIV
510 520 530 540 550
LTVAKKATTI QLGNYIGRNS TEVISELKQK KVPENLIKIE EEESSESEPG
560 570 580 590 600
TIMKQSPGAG TTYDVSKPTQ IVLTVAKKVT SVAMPSYIGS SLEFTKNNLI
610 620 630 640 650
QIVGIKEANI EVVEVTTAPA GSAEGMVVEQ SPRAGEKVDL NKTRVKISIY
KPKTTSATP
SEQUENCE LENGTH - 659
CURRENT FIELD STATUS

CURRENT TRIAL STATUS
Phase 1
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Immunisation of mice with StkP
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Immunisation of combination vaccine containing StkP, PsaA, and PcsB

IMMUNE RESPONSE GENERATED
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In vitro studies show StkP is accessible on the pneumococcal cell surface to antibodies in humans and mice.
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StkP is shown to protect against lethal forms of sepsis and pneumonia.
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Sktp has been shown to be safe to use, well tolerated within the body, and highly immunogenic in clinical trials when used in a SktP-PsaA-PcsB combination vaccine in adults. This was measured using Immunoglobulin G responses specific to each protein.

MECHANISM OF VIRULENCE
• Surface-exposed protein, therefore, increases virulence as it plays a key role in the infectious process of Streptococcus pneumoniae.
• StkP is a non-classical protein that has strong links to the pathogenicity of Streptococcus pneumoniae as can influence the colonisation and spreading of bacteria.
• StkP has been proven to increase virulence in mouse models infected with S. pneumoniae.
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